In public transport, Route 5 may refer to:

• Line 5 Blue (Montreal Metro)

• 5 (New York City Subway service)
• 5 (New Jersey bus), a New Jersey Transit bus route
• Route 5 (MTA Maryland), a bus route in Baltimore, Maryland
• 5 (Los Angeles Railway), a line operated by the Los Angeles Railway from 1931 to 1958
• 5 Fulton, a bus route in San Francisco

• Mexico City Metro Line 5

• Barcelona Metro line 5
• Line 5 (Madrid Metro)

• London Buses route 5

• Citybus Route 5, a bus route in Hong Kong

• Seoul Subway Line 5

• Melbourne tram route 5