On Her Majesty's Secret Service may refer to:

• On Her Majesty's Secret Service (novel), a 1963 James Bond novel by Ian Fleming
• On Her Majesty's Secret Service (film), a 1969 film adaptation of the novel by Peter R. Hunt
  • On Her Majesty's Secret Service (soundtrack), the soundtrack from the film by John Barry, including the title theme
• On Her Majesty's Secret Service (Propellerheads song), from the 1998 Propellerheads album Decksandrumsandrockandroll

• Secret Service (disambiguation)
• Her Majesty's Secret Service (now His Majesty’s Secret Service) or MI6, the British intelligence agency
• On His Majesty's Service, formerly On Her Majesty's Service, franking (postage) on official documents in the United Kingdom and Commonwealth countries
• On His Majesty's Secret Service, a 2009 Hong Kong film
• On Her Majesty's Occult Service, a 2007 novel by Charles Stross
• "On Her Majesty's Sewer Service", a television episode featuring Mario