Missio may refer to:

• Missio, public facing name of Pontifical Mission Societies, the Catholic Church's official charity for overseas mission

• Egone Missio Fondo Egone Missio Archives
• Stefano Missio (1972) Italian filmmaker

• Missio (duo), US electronic duo formed in 2014
• Missió, late 1980s Hungarian heavy metal band, see Impulse (band)

• Missio Dei, Latin theological term for the "sending of God"
• Honesta missio, honorable discharge from the military service in the Roman Empire
• Redemptoris missio (Latin: The Mission of the Redeemer), subtitled On the permanent validity of the Church's missionary mandate, encyclical