James (or Jim or Jamie) Mackenzie (or McKenzie) may refer to:

• Jim Mackenzie (American football) (1930–1967), football coach
• James McKenzie (boxer) (1903–1931), British boxer
• Jim McKenzie (footballer) (1877–?), Scottish-born footballer who played for Burton Swifts and Southampton in the 1890s
• Jamie McKenzie (footballer, born 1980), Scottish-born football midfielder whose clubs have included Partick Thistle and Albion Rovers
• Jamie McKenzie (footballer, born 1986), Scottish-born football defender who has played for clubs in Scotland, Ireland and Cyprus
• Jim McKenzie (ice hockey) (born 1969), Canadian ice hockey player whose teams included Hartford Whalers, Phoenix Coyotes and New Jersey Devils
• James MacKenzie (rugby union) (1886–1963), Scottish international rugby union player

• James MacKenzie (VC) (1889–1914), Scottish recipient of the Victoria Cross
• Sir James Mackenzie (cardiologist) (1853–1925), Scottish cardiologist
• James A. McKenzie (1840–1904), U.S. Representative from Kentucky
• James A. McKenzie (Wisconsin politician), American businessman and politician
• James Mckenzie (outlaw) (1820–?), Scottish-born New Zealand outlaw
• James Mackenzie (actor) (born 1979), Scottish actor who presents the children's game show Raven
• James McKenzie (Manitoba politician) (1854–1936), politician in Manitoba, Canada
• James McKenzie (Australian politician) (1867–1939)
• James Cameron Mackenzie (1852–1931), American educator
• James Hewat McKenzie (1869–1929), British parapsychologist
• Kenzie (James MacKenzie, born 1986), British rapper
• James Hutton Mackenzie (1849–1949), New Zealand Presbyterian minister
• James Stuart-Mackenzie (c. 1719–1800), Scottish politician
• James B. McKenzie (1926–2002), American theater producer
• James George Mackenzie, Royal Navy officer and governor of the Falkland Islands
• James Mackenzie, Lord Royston (1671–1744), Scottish judge