Dark Seed or Darkseed may refer to:

• Dark Seed (video game), a 1992 horror adventure game
• Dark Seed II, a 1995 sequel to the 1992 adventure game
• Darkseed (band), a German gothic metal band formed in 1992
• Dark Seed, an Australian indigenous music group managed by Vibe Australia
• Dark Seed, a DeBeers story by V. C. Andrews
• The Dark Seed, a book based on the role-playing game Savage Worlds

• Darkseid, a fictional character in DC Comics