Needle in a haystack may refer to:

• "Needle in a Haystack" (House), an episode of the TV series House
• "Needle in a Haystack", an episode of the TV series Mythbusters
• "Needle in a Haystack", a 1964 pop song record by The Velvelettes
• Terms in a two-way string-matching algorithm

• Needle in the Haystack, a 1953 Brazilian comedy film