Five-time defending champion Diede de Groot^[1] defeated Yui Kamiji in the final, 6–2, 6–2 to win the women's singles wheelchair tennis title at the 2023 US Open.^[2] With the win, de Groot completed the Grand Slam for a third consecutive year, and won a 12th consecutive major singles title (an all-time record in any tennis discipline).^[3] This marked the seventh consecutive year where de Groot and Kamiji contested the final.

	First round							Quarterfinals							Semifinals							Final
	1	Diede de Groot	6	6
	WC	Pauline Déroulède	2	0				1	D de Groot	6	6
		Lucy Shuker	4	6	6				L Shuker	2	0
		Manami Tanaka	6	3	4										1	D de Groot	6	6
	4	Momoko Ohtani	6	6											4	M Ohtani	3	1
		Zhu Zhenzhen	0	2				4	M Ohtani	6	6
		Kgothatso Montjane	6	6					K Montjane	2	4
	WC	María Florencia Moreno	2	1																		1	D de Groot	6	6
		Aniek van Koot	6	6																		2	Y Kamiji	2	2
		Lizzy de Greef	0	3					A van Koot	4	3
		Shiori Funamizu	1	1				3	J Griffioen	6	6
	3	Jiske Griffioen	6	6											3	J Griffioen	0	0r
		Angélica Bernal	6	6											2	Y Kamiji	6	0
		Katharina Krüger	4	1					A Bernal	3	2
		Dana Mathewson	5	1				2	Y Kamiji	6	6
	2	Yui Kamiji	7	6

• Draw