Marjolein Buis defeated Sabine Ellerbrock in the final, 6–3, 6–4 to win the women's singles wheelchair tennis title at the 2016 French Open.

Jiske Griffioen was the defending champion, but was defeated in the quarterfinals by Jordanne Whiley.

	Quarterfinals							Semifinals							Final
	1	Jiske Griffioen	0	6	3
		Jordanne Whiley	6	2	6
									Jordanne Whiley	3	6	4
									Sabine Ellerbrock	6	1	6
		Sabine Ellerbrock	6	6
	WC	Charlotte Famin	4	2
																Sabine Ellerbrock	3	4
																Marjolein Buis	6	6
		Lucy Shuker	1	6	2
		Marjolein Buis	6	4	6
									Marjolein Buis	77	63	6
								2	Yui Kamiji	65	77	1
		Aniek van Koot	6	0	3
	2	Yui Kamiji	2	6	6

• Entry list
• Draw