The Seymour line is a regional passenger rail service operated by V/Line in Victoria, Australia. It serves 13 stations towards its terminus in the regional city of Seymour. It is the fifth most-used regional rail service in Victoria (behind the Gippsland, Bendigo, Ballarat, and Geelong services), carrying 1.75 million passengers in the 2023–2024 financial year.^[1]

Beyond Seymour, the Shepparton line continues further north to Shepparton; and the Albury line continues north-east to its terminus at Albury, just over the border in New South Wales.

V/Line operates hourly passenger services from Seymour to Melbourne, using Sprinter and VLocity railcars. Five return services operate to Shepparton on weekdays; three return services operate on weekends; all are operated by the VLocity. Until 31 October 2022, services to Shepparton were operated by the N set, which included a buffet and first-class seating, this is no longer available as the VLocity does not have catering facilities and first class seating onboard. H type carriages were also used on the line until the introduction of the VLocity on the Shepparton line. Like the VLocity, it did not have a buffet and first class onboard.

Services generally stop all stations between Shepparton and Donnybrook in both directions. Some services run express through Donnybrook, Heathcote Junction and Tallarook. Some services stop at Essendon and North Melbourne on weekdays; all services stop at Broadmeadows. At metropolitan stations, services towards Seymour and Shepparton only stop to pick up passengers, and services towards Southern Cross only stop to allow passengers to alight.

Platform 1 connects to the Albury Line, which uses standard gauge (SG). Platform 2 connects the Seymour and Shepparton services, which utilise the broad gauge (BG) track.

Tocumwal railway line
Legend
km Multiple lines via Richmond or Jolimont City Loop 0.0 Flinders Street ●●●●●●●●●●●● 1.4 Parliament ●●●●●●●●● 2.7 Melbourne Central 3.3 Flagstaff 1.2 Southern Cross ● City Loop North East SG & Western SG lines to Albury, Sydney & Adelaide 2.9 North Melbourne ●●●●●●● 3.1 Dynon Road Upfield line to Upfield 3.7 CityLink 3.7 Moonee Ponds Creek Multiple lines via Footscray 4.8 Kensington 5.4 Racecourse Road 5.5 Newmarket ■ Flemington Racecourse line to Flemington Racecourse 6.8 Ascot Vale Road 7.0 Ascot Vale 7.4 Maribyrnong Road 8.1 Moonee Ponds 9.2 Essendon ●●■ 9.5 Mount Alexander Road 10.4 Glenbervie 11.0 Strathmore 11.3 Pascoe Vale Road 11.7 Moonee Ponds Creek 11.8 Bell Street 11.9 CityLink 12.5 Pascoe Vale 14.1 Oak Park 15.6 Glenroy North East SG line to Southern Cross & Albion–Jacana line to Albion 16.7 Jacana 16.7 Western Ring Road 17.8 Camp Road 18.1 Broadmeadows ●●● Broadstore Siding (demolished) 20.5 Coolaroo Upfield line to Flinders Street 22.9 Somerton Road 23.0 Somerton (demolished) 23.3 Somerton Yard | Roxburgh Park 27.3 Craigieburn ●● 27.6 Hume Highway / Sydney Road 28.0 end of overhead 28.7 Hume Freeway 31.0 end of Metro area 32.6 Merri Creek 34.4 Donnybrook Merri Creek 43.0 Beveridge 48.7 Wallan 51.9 Lightwood (demolished) 54.7 Heathcote Junction Heathcote line to Bendigo 56.5 Wandong 58.0 Hume Freeway 59.9 Mathieson's Siding (demolished) 64.7 Kilmore East 67.2 Kilmore East Quarry Siding 76.4 Broadford 78.5 McDougall's Siding (demolished) 86.9 Lowry's Siding (demolished) 89.7 Hume Freeway 91.4 Tallarook Mansfield line to Mansfield 95.7 Dysart 97.6 Goulburn River 99.9 Seymour ●● Hume Freeway 108.1 Gravelside Siding (demolished) 110.6 Mangalore (demolished) North East SG line to Albury & Sydney 119.20 Tabilk Goulburn Valley Freeway 128.11 Nagambie ● Goulburn Valley Freeway 135.90 Wahring Goulburn Valley Freeway Rushworth line to Colbinabbin 149.24 Murchison East ● 157.90 Arcadia Goulburn River 166.05 Toolamba Toolamba-Echuca line to Echuca 179.00 Mooroopna ● Goulburn River Broken River 184.84 Shepparton ● Dookie railway line Pine Lodge Cosgrove Dookie Katamatite railway line to Katamatite 191.90 Congupna 199.00 Tallygaroopna 208.30 Wunghnu Broken Creek Goulburn Valley Highway 215.20 Numurkah Picola line to Picola 215.70 RMSP 85 225.80 Katunga 227.80 RMSP 86 231.70 RMSP 81 Goulburn Valley Highway 237.90 Strathmerton Cobram line to Cobram Goulburn Valley Highway 241.60 Mywee 250.30 Tocumwal Bridge 304.0 Bridge over Murray River Victoria NSW border 227.80 Tocumwal Tocumwal line (NSW) to Narrandera
broad gauge standard gauge dual gauge

• V/Line website