Route 140 or Highway 140 may refer to:

• Hamilton Highway

• New Brunswick Route 140
• Ontario Highway 140
• Prince Edward Island Route 140

• National Route 140

• Bundesautobahn 140

• National Highway 140 (India)

• Japan National Route 140
• Fukuoka Prefectural Route 140
• Nara Prefectural Route 140

• Malaysia Federal Route 140

• A140 road
• B140 road

• Interstate 140 (two current highways)
• U.S. Route 140 (former)
• Alabama State Route 140
• Arkansas Highway 140
• California State Route 140
• Colorado State Highway 140
• Connecticut Route 140
• Florida State Road 140 (pre-1945) (former)
  • Florida State Road 140A (pre-1945) (former)
  • County Road 140 (Madison County, Florida)
• Georgia State Route 140
• Illinois Route 140
• Indiana State Road 140
• Iowa Highway 140
• K-140 (Kansas highway)
• Kentucky Route 140
• Louisiana Highway 140
• Maine State Route 140
• Maryland Route 140
• Massachusetts Route 140
• M-140 (Michigan highway)
• Missouri Route 140 (former)
• Nevada State Route 140
• New Hampshire Route 140
• New Jersey Route 140
• New Mexico State Road 140
• New York State Route 140
  • County Route 140 (Broome County, New York)
  • County Route 140 (Erie County, New York)
  • County Route 140 (Fulton County, New York)
  • County Route 140 (Montgomery County, New York)
  • County Route 140 (Niagara County, New York)
  • County Route 140 (Onondaga County, New York)
  • County Route 140 (Rensselaer County, New York)
  • County Route 140 (Wayne County, New York)
• North Carolina Highway 140
• Ohio State Route 140
• Oregon Route 140
• Tennessee State Route 140
• Texas State Highway 140
  • Texas State Highway Loop 140 (former)
  • Texas State Highway Spur 140
  • Farm to Market Road 140
• Utah State Route 140
  • Utah State Route 140 (pre-1969) (former)
• Vermont Route 140
• Virginia State Route 140
  • Virginia State Route 140 (pre-1933) (former)
  • Virginia State Route 140 (1933-1949) (former)
• Washington State Route 140 (former)
• West Virginia Route 140
• Wisconsin Highway 140

Territories

• Puerto Rico Highway 140