Millbrook may refer to:

• Millbrook, Victoria
• Millbrook, Western Australia, a locality of the City of Albany

• Millbrook First Nation, including the community Millbrook 27, Nova Scotia
• Millbrook, Ontario

• Millbrook Resort, a luxury resort near Queenstown

• Millbrook, Bedfordshire, a village
  • Home to the Millbrook Proving Ground
• Millbrook, Cornwall, a village
  • Home to Millbrook AFC, who play at Jenkins Park
• Millbrook, Axminster, an area of Axminster, Devon
• Millbrook, North Molton, a location in Devon
• Millbrook, Greater Manchester, an area of Stalybridge
• Millbrook, Southampton, a district of Southampton, England

• Millbrook, Alabama
• Millbrook, Illinois
• Millbrook, Kansas
• Millbrook, Missouri
• Millbrook, New Jersey
• Millbrook, New York
• Millbrook, North Carolina
• Millbrook, Ohio
• Millbrook, Pennsylvania
• Millbrook, West Virginia
• Millbrook, Wyoming

• Millbrook, common designation for the Hitchcock Estate in Millbrook, New York, associated with Timothy Leary
• Millbrook Commonwealth Action Programme, a programme of the Commonwealth of Nations agreed at Millbrook Resort
• Millbrook (Crewe, Virginia), listed on the National Register of Historic Places in Nottoway County, Virginia
• Millbrook High School (disambiguation)
• Millbrook, Indiana, fictional town in the 2005 film A History of Violence
• Millbrook Primary School, Grove, Oxfordshire, England
• Millbrook, Toowoomba, a heritage house in Queensland, Australia
• Millbrook Winery, a winery and restaurant at Jarrahdale, in the Perth Hills wine region of Western Australia
• Millbrook Press, an American children's book publisher

• Mill Brook (disambiguation)
• Molenbeek (disambiguation)