In enzymology, a glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (EC 1.2.1.59) is an enzyme that catalyzes the chemical reaction

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ 3-phospho-D-glyceroyl phosphate + NAD(P)H + H⁺

The four substrates of this enzyme are D-glyceraldehyde 3-phosphate, phosphate, NAD⁺, and NADP⁺, whereas its four products are 3-phospho-D-glyceroyl phosphate, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of a donor with NAD+ or NADP+ as an acceptor. The systematic name of this enzyme class is D-glyceraldehyde 3-phosphate:NAD(P)+ oxidoreductase (phosphorylating). Other names in common use include triosephosphate dehydrogenase (NAD(P)) (phosphorylating), and glyceraldehyde-3-phosphate dehydrogenase (NAD(P)) (phosphorylating).

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2CZC.

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