Crossover junction endonuclease EME1 is an enzyme that in humans is encoded by the EME1 gene.[5][6] It forms a complex with MUS81 which resolves Holliday junctions. In mammalian cells the EME1/MUS81 protein complex is redundant for DNA damage repair with GEN1 endonuclease.[7] In mice, EME1/MUS81 and GEN1 redundantly contribute to Holliday junction processing. When homozygous mutations of Gen1 and Eme1 were combined in mice the result was synthetic lethality at an early embryonic stage.[7] Homozygosity for Gen1 mutations did not cause a DNA repair deficiency in mice. But when mice were both homozygous mutant for Gen1 and also heterozyous for an Emc1 mutation, they showed increased sensitivity to DNA damaging agents. This finding, indicated a redundant role of GEN1 and EME1 in DNA repair. Gen1 and Emc1 were also shown to have redundant roles in meiotic recombination.[7]
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000154920 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000039055 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Ciccia A, Constantinou A, West SC (July 2003). "Identification and characterization of the human mus81-eme1 endonuclease". The Journal of Biological Chemistry. 278 (27): 25172–25178. doi:10.1074/jbc.M302882200. PMID 12721304.
- ^ "Entrez Gene: EME1 essential meiotic endonuclease 1 homolog 1 (S. pombe)".
- ^ a b c Wang X, Wang H, Guo B, Zhang Y, Gong Y, Zhang C, et al. (October 2016). "Gen1 and Eme1 Play Redundant Roles in DNA Repair and Meiotic Recombination in Mice". DNA and Cell Biology. 35 (10): 585–590. doi:10.1089/dna.2015.3022. PMC 6445196. PMID 27383418.
Further reading
- Oğrünç M, Sancar A (June 2003). "Identification and characterization of human MUS81-MMS4 structure-specific endonuclease". The Journal of Biological Chemistry. 278 (24): 21715–21720. doi:10.1074/jbc.M302484200. PMID 12686547.
- Abraham J, Lemmers B, Hande MP, Moynahan ME, Chahwan C, Ciccia A, et al. (November 2003). "Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells". The EMBO Journal. 22 (22): 6137–6147. doi:10.1093/emboj/cdg580. PMC 275438. PMID 14609959.
- Blais V, Gao H, Elwell CA, Boddy MN, Gaillard PH, Russell P, McGowan CH (February 2004). "RNA interference inhibition of Mus81 reduces mitotic recombination in human cells". Molecular Biology of the Cell. 15 (2): 552–562. doi:10.1091/mbc.E03-08-0580. PMC 329235. PMID 14617801.
- Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, et al. (August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–12135. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
- Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–1178. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
- Hiyama T, Katsura M, Yoshihara T, Ishida M, Kinomura A, Tonda T, et al. (2006). "Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells". Nucleic Acids Research. 34 (3): 880–892. doi:10.1093/nar/gkj495. PMC 1360746. PMID 16456034.
- Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (November 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–648. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.