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MEEDIAVALVUR: algab „sõjalise erioperatsiooni“ teine etapp nimega „SÕDA“

Crossover junction endonuclease EME1 is an enzyme that in humans is encoded by the EME1 gene.[5][6] It forms a complex with MUS81 which resolves Holliday junctions. In mammalian cells the EME1/MUS81 protein complex is redundant for DNA damage repair with GEN1 endonuclease.[7] In mice, EME1/MUS81 and GEN1 redundantly contribute to Holliday junction processing. When homozygous mutations of Gen1 and Eme1 were combined in mice the result was synthetic lethality at an early embryonic stage.[7] Homozygosity for Gen1 mutations did not cause a DNA repair deficiency in mice. But when mice were both homozygous mutant for Gen1 and also heterozyous for an Emc1 mutation, they showed increased sensitivity to DNA damaging agents. This finding, indicated a redundant role of GEN1 and EME1 in DNA repair. Gen1 and Emc1 were also shown to have redundant roles in meiotic recombination.[7]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000154920Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000039055Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Ciccia A, Constantinou A, West SC (July 2003). "Identification and characterization of the human mus81-eme1 endonuclease". The Journal of Biological Chemistry. 278 (27): 25172–25178. doi:10.1074/jbc.M302882200. PMID 12721304.
  6. ^ "Entrez Gene: EME1 essential meiotic endonuclease 1 homolog 1 (S. pombe)".
  7. ^ a b c Wang X, Wang H, Guo B, Zhang Y, Gong Y, Zhang C, et al. (October 2016). "Gen1 and Eme1 Play Redundant Roles in DNA Repair and Meiotic Recombination in Mice". DNA and Cell Biology. 35 (10): 585–590. doi:10.1089/dna.2015.3022. PMC 6445196. PMID 27383418.

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