This is a list of people who have served as Custos Rotulorum of Middlesex.

• Sir Roger Cholmley bef. 1544 – aft. 1547
• Edward Hastings, Baron Hastings of Loughborough bef. 1558 – 1571
• Richard Goderick bef. 1562 – bef. 1564
• Sir Thomas Wroth bef. 1564–1573
• Sir Gilbert Gerard 1573–1593
• Sir John Fortescue c. 1594–1607
• Sir Thomas Lake c. 1608–1619
• Sir Thomas Edmondes 1619–1639
• Sir Henry Vane 1639–1642
• Sir Peter Wyche 1642–1643
• Sir Edward Nicholas 1643–1646
• Interregnum
• Sir Edward Nicholas 1660–1669
• William Craven, 1st Earl of Craven 1669–1689
• John Holles, 4th Earl of Clare 1689–1692
• William Russell, 1st Duke of Bedford 1692–1700
• Lord Edward Russell 1700–1701
• Wriothesley Russell, 2nd Duke of Bedford 1701–1711
• John Sheffield, 1st Duke of Buckingham and Normanby 1711–1714
• Thomas Pelham-Holles, 1st Duke of Newcastle 1714–1762
• Hugh Percy, 1st Duke of Northumberland 1762–1786
• vacant
• Henry Dundas 1793–1794
• William Bentinck, Marquess of Titchfield 1794–1802

For later custodes rotulorum, see Lord Lieutenant of Middlesex.

• Institute of Historical Research - Custodes Rotulorum 1544-1646
• Institute of Historical Research - Custodes Rotulorum 1660-1828