Sixteen or 16 may refer to:

• 16 (number)
• one of the years 16 BC, AD 16, 1916, 2016

• Pathinaaru or Sixteen, a 2010 Tamil film
• Sixteen (1943 film), a 1943 Argentine film directed by Carlos Hugo Christensen
• Sixteen (2013 Indian film), a 2013 Hindi film
• Sixteen (2013 British film), a 2013 British film by director Rob Brown

• The Sixteen, an English choir
• 16 (band), a sludge metal band
• Sixteen (Polish band), a Polish band

• 16 (Robin album), a 2014 album by Robin
• 16 (Madhouse album), a 1987 album by Madhouse
• Sixteen (album), a 1983 album by Stacy Lattisaw
• Sixteen , a 2005 album by Shook Ones
• 16, a 2020 album by Wejdene

• "16" (Sneaky Sound System song), 2009
• "Sixteen" (Thomas Rhett song), 2017
• "Sixteen" (Ellie Goulding song), 2019
• "Six7een", by Hori7on, 2023
• "16", by Craig David from Following My Intuition, 2016
• "16", by Green Day from 39/Smooth, 1990
• "16", by Highly Suspect from MCID, 2019
• "Sixteen", by Buzzcocks from Another Music in a Different Kitchen, 1978
• "Sixteen", by Demon Hunter from Storm the Gates of Hell, 2007
• "Sixteen", by The Flaws from Achieving Vagueness, 2007
• "Sixteen", by Funeral for a Friend from The Young and Defenceless and Welcome Home Armageddon, 2010
• "Sixteen", by Iggy Pop from Lust for Life, 1977
• "Sixteen", by The Indelicates, 2007
• "Sixteen", by Le Tigre from This Island, 2004
• "Sixteen", by No Doubt from Tragic Kingdom, 1995
• "Christine Sixteen", by Kiss, 1977

• Sixteen, Kentucky, an American unincorporated community in Perry County
• Sixteen, Montana, an American unincorporated community in Meagher County

• 16 (magazine), a fan magazine marketed to adolescents
• 6teen, a television series distributed by Teletoon
• Number 16 (spider), world's longest-lived spider
• Sixteen (card game), a card game published by Alpine Games
• Sixteen (Moesha), a 1996 episode of the comedy-drama series Moesha
• Sixteen (restaurant), on the 16th floor of Trump International Hotel and Tower, Chicago
• Sixteen (TV series), a 2015 South Korean music competition reality show
• Sulfur, a nonmetal in the periodic table
• 16 Psyche, a main-belt asteroid
• Rover 16, a mid-size car
• Renault 16, a hatchback
• Hillman 16, an executive car

• List of highways numbered 16