Route 396, or Highway 396, may refer to:

• Manitoba Provincial Road 396
• Saskatchewan Highway 396 (former)

• Japan National Route 396

• Arkansas Highway 396 (former)
• Maryland Route 396
  • Maryland Route 396A
• Nevada State Route 396
• New York:
  • New York State Route 396
  • County Route 396 (Erie County, New York)
• Puerto Rico Highway 396
• Tennessee State Route 396
• Texas:
  • Texas State Highway Loop 396
  • Farm to Market Road 396
  • Urban Road 396
• Virginia State Route 396