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De novo (Latin, lit. 'of new', used in English to mean 'from the beginning', 'anew') may refer to:

Science and computers

De novo mutation, a new germline mutation not inherited from either parent
De novo protein design, the creation of a protein sequence that is not based on existing, natural sequences
De novo protein structure prediction, the prediction of a protein's 3D structure, based only on its sequence
De novo synthesis of complex molecules from simple molecules in chemistry
De novo transcriptome assembly, the method of creating a transcriptome without a reference genome, using de novo sequence assembly
De novo gene birth, the emergence of genes from non-coding sequence
De novo domestication, the domestication of new species for human use
De novo assembly in genomics
De Novo classification, a pathway to classify new medical devices provided by the US Federal Food, Drug, and Cosmetic Act
Denovo, a supercomputer project that simulates nuclear reactions; see Titan (supercomputer) § Projects