Sapho may refer to:

• Sapho (damselfly), genus of insects
• SAPHO syndrome, chronic disease with synovitis, acne, pustulosis, hyperostosis and osteitis

• Madeleine de Scudéry (1607–1701), French writer who used the pseudonym Sapho
• Sapho (singer) (born 1950), French singer

• Sapho (Gounod), 1851 opera by Charles Gounod
• Sapho (novel) (1884), by Alphonse Daudet
• Sapho (Massenet), 1897 opera by Jules Massenet, based on Daudet's novel
• Sapho (play), 1900 play by Clyde Fitch
• Queen Sapho, a main character in the 1584 Elizabethan play Sapho and Phao
• Sapho (1913 film), a lost 1913 silent film feature drama
• Sapho (1917 film), a 1917 American silent drama film
• Sapho (1934 film), a 1934 French drama film
• Sapho juice, a stimulant drug in the fictional Dune universe

• Sappho (6th century BC), Greek lyric poet
• Sappho (disambiguation)
• Saffo (disambiguation)