The Roman Catholic Diocese of Matagalpa (erected 19 December 1924) is a suffragan of the Archdiocese of Managua.

• Isidro Carrillo y Salazar (1924–1931)
• Vicente Alejandro González y Robleto (1932–1938). appointed Coadjutor Archbishop of Managua
• Isidro Augusto Oviedo y Reyes (1939–1946), appointed Bishop of León en Nicaragua
• Octavio José Calderón y Padilla (1946–1970)
• Julián Luis Barni Spotti, O.F.M. (1970–1982), appointed Bishop of León en Nicaragua
• Carlos José Santi Brugia, O.F.M. (1982–1991)
• Leopoldo José Brenes Solórzano (1991–2005), appointed Archbishop of Managua
• Jorge Solórzano Pérez (2005–2010), appointed Bishop of Granada
• Rolando José Álvarez Lagos (since 2011)

• Miguel Obando Bravo, S.D.B. (1968-1970), appointed Archbishop of Managua (Cardinal in 1985)

• Pedro Lisímaco de Jesús Vílchez Vílchez, appointed Bishop of Jinotega in 1982

• Catholic Church in Nicaragua

• "Diocese of Matagalpa". Catholic-Hierarchy. Retrieved 2007-03-03. ^{[self-published]}