Guadalupe District may refer to:
Peru
- Guadalupe District, Pacasmayo, in Pacasmayo province, La Libertad region
Costa Rica
- Guadalupe District, Zarcero, in the canton of Zarcero, Alajuela province
- Guadalupe o Arenilla District, Cartago, in the canton of Cartago, Cartago province
- Guadalupe District, Goicoechea, in the canton of Goicoechea, San José province
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