The 2021 Macha Lake Open was a professional women's tennis tournament played on outdoor clay courts. It was the nineteenth edition of the tournament which was part of the 2021 ITF Women's World Tennis Tour. It took place in Staré Splavy, Czech Republic between 14 and 20 June 2021.^[1]

Country	Player	Rank1	Seed
SVK	Kristína Kučová	150	1
TUR	Çağla Büyükakçay	179	2
USA	Allie Kiick	181	3
HUN	Réka Luca Jani	185	4
SVK	Rebecca Šramková	193	5
AUT	Julia Grabher	195	6
PAR	Verónica Cepede Royg	199	7
ROU	Gabriela Talabă	211	8

• ¹ Rankings are as of 31 May 2021.

The following players received wildcards into the singles main draw:

• Nikola Bartůňková
• Sára Bejlek
• Linda Nosková
• Darja Viďmanová

The following player received entry using a protected ranking:

• Destanee Aiava
• Alexandra Dulgheru

The following players received entry from the qualifying draw:

• Irene Burillo Escorihuela
• Miriam Kolodziejová
• Johana Marková
• İpek Öz
• Andreea Prisăcariu
• Dominika Šalková
• Tereza Smitková
• Kimberley Zimmermann

• Zheng Qinwen def. Aleksandra Krunić, 7–6^(7–5), 6–3

• Valentini Grammatikopoulou / Richèl Hogenkamp def. Amina Anshba / Anastasia Dețiuc, 6–3, 6–4

• 2021 Macha Lake Open at ITFtennis.com
• Official website