Western University may refer to:

• University of Western Ontario, a public university in London, Ontario, Canada

• Western Illinois University, a public university in Macomb, Illinois, United States
• Western Connecticut State University, a public university in Danbury, Connecticut
• Western Reserve University, a former name of Case Western Reserve University, a private research university in Cleveland, Ohio
• Western Washington University, a public university located in Bellingham, Washington, United States
• Western University (Kansas), a historically black college in Quindaro, Kansas, United States from 1865 to 1943
• Western University of Health Sciences, a private graduate-level university in Pomona, California, United States
• Western University of Pennsylvania, a former name of the University of Pittsburgh from 1819 to 1908
• Western Colorado University, a public university in Gunnison, Colorado

• Western Caspian University, a private university in Baku, Azerbaijan

• Midwestern University, Illinois and Arizona