The enzyme trans-L-3-hydroxyproline dehydratase (EC 4.2.1.77) catalyzes the chemical reaction

trans-L-3-hydroxyproline Δ₁-pyrroline 2-carboxylate + H₂O

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is trans-L-3-hydroxyproline hydro-lyase (Δ₁-pyrroline-2-carboxylate-forming). This enzyme is also called trans-L-3-hydroxyproline hydro-lyase.

• Ramaswamy SG (1983). "Conversion of 3-hydroxyproline to proline in the rat requires reduced pyridine-nucleotides". Fed. Proc. 42: 2232.
• Visser WF, Verhoeven-Duif NM, de Koning TJ (June 2012). "Identification of a human trans-3-hydroxy-L-proline dehydratase, the first characterized member of a novel family of proline racemase-like enzymes". The Journal of Biological Chemistry. 287 (26): 21654–62. doi:10.1074/jbc.m112.363218. PMC 3381129. PMID 22528483.