A toothbrush is an oral hygiene instrument.

Toothbrush may also refer to:

• Toothbrush moustache, a moustache style
• Toothbrush (album) by Dr. Dog, 2002
• "Toothbrush" (song), a 2016 song by DNCE
• "Toothbrush" (The Apprentice), a 2022 television episode
• "Toothbrush" (Maalaala Mo Kaya), a 2016 episode about Leni Robredo