In enzymology, a salicylaldehyde dehydrogenase (EC 1.2.1.65) is an enzyme that catalyzes the chemical reaction

salicylaldehyde + NAD⁺ + H₂O salicylate + NADH + 2 H⁺

The 3 substrates of this enzyme are salicylaldehyde, NAD⁺, and H₂O, whereas its 3 products are salicylate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is salicylaldehyde:NAD+ oxidoreductase. This enzyme participates in naphthalene and anthracene degradation.

• Eaton RW, Chapman PJ (1992). "Bacterial metabolism of naphthalene: construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions". J. Bacteriol. 174 (23): 7542–54. PMC 207464. PMID 1447127.