In enzymology, a pseudouridine kinase (EC 2.7.1.83) is an enzyme that catalyzes the chemical reaction

ATP + pseudouridine ADP + pseudouridine 5'-phosphate

Thus, the two substrates of this enzyme are ATP and pseudouridine, whereas its two products are ADP and pseudouridine 5'-phosphate.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:pseudouridine 5'-phosphotransferase. This enzyme is also called pseudouridine kinase (phosphorylating). This enzyme participates in pyrimidine metabolism.

• Solomon LR, Breitman TR (1971). "Pseudouridine kinase of escherichia coli: a new enzyme". Biochem. Biophys. Res. Commun. 44 (2): 299–304. doi:10.1016/0006-291X(71)90599-7. PMID 4334133.