In enzymology, a procollagen galactosyltransferase (EC 2.4.1.50) is an enzyme that catalyzes the chemical reaction

UDP-galactose + procollagen 5-hydroxy-L-lysine UDP + procollagen 5-(D-galactosyloxy)-L-lysine

Thus, the two substrates of this enzyme are UDP-galactose and procollagen 5-hydroxy-L-lysine, whereas its two products are UDP and procollagen 5-(D-galactosyloxy)-L-lysine.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-galactose:procollagen-5-hydroxy-L-lysine D-galactosyltransferase. Other names in common use include hydroxylysine galactosyltransferase, collagen galactosyltransferase, collagen hydroxylysyl galactosyltransferase, UDP galactose-collagen galactosyltransferase, uridine diphosphogalactose-collagen galactosyltransferase, and UDPgalactose:5-hydroxylysine-collagen galactosyltransferase. This enzyme participates in lysine degradation.

Structure and functons of procollagen galactosyltransferase 1

Procollagen galactosyltransferase 1 (GT251), encoded by the COLGALT1 gene, plays a crucial role in lysyl O-linked glycosylation and the maturation of collagen.[1] GT251 consists of two galactosyltransferase domains (GalT-N and GalT-C) and is stabilized in a dimeric form.[2] The GT251 dimer can further associate with LH3 (encoded by PLOD3) to form a heterotetrameric complex, known as the KOGG complex (Lysyl Hydroxylation-Galactosylation-Glucosylation complex).[2] Within this complex, three key enzymatic reactions in lysine O-linked glycosylation are coordinately catalyzed by LH3 and GT251, ensuring proper collagen modification and structural integrity.

References

  1. ^ Schegg, Belinda; Hülsmeier, Andreas J.; Rutschmann, Christoph; Maag, Charlotte; Hennet, Thierry (2009-02-01). "Core Glycosylation of Collagen Is Initiated by Two β(1- O )Galactosyltransferases". Molecular and Cellular Biology. 29 (4): 943–952. doi:10.1128/MCB.02085-07. ISSN 1098-5549. PMC 2643808. PMID 19075007.
  2. ^ a b Peng, Junjiang; Li, Wenguo; Yao, Deqiang; Xia, Ying; Wang, Qian; Cai, Yan; Li, Shaobai; Cao, Mi; Shen, Yafeng; Ma, Peixiang; Liao, Rijing; Zhao, Jie; Qin, An; Cao, Yu (2025-03-11). "The structural basis for the human procollagen lysine hydroxylation and dual-glycosylation". Nature Communications. 16 (1). doi:10.1038/s41467-025-57768-9. ISSN 2041-1723. PMC 11897130.
  • Bosmann HB, Eylar EH (1968). "Glycoprotein biosynthesis: the biosynthesis of the hydroxylysine-galactose linkage in collagen". Biochem. Biophys. Res. Commun. 33 (2): 340–6. doi:10.1016/0006-291X(68)90790-0. PMID 5722225.
  • Kivirikko KI; Myllyla R (1979). Hall, D.A.; Jackson, D.S. (eds.). "Collagen glycosyltransferases". International Review of Connective Tissue Research. 8. New York: Academic Press: 23–72. doi:10.1016/B978-0-12-363708-6.50008-4. ISBN 9780123637086. PMID 389860.


Allikas: http://en.wikipedia.org/wiki/Procollagen_galactosyltransferase
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