In enzymology, a pimeloyl-CoA dehydrogenase (EC 1.3.1.62) is an enzyme that catalyzes the chemical reaction

pimeloyl-CoA + NAD⁺ 6-carboxyhex-2-enoyl-CoA + NADH + H⁺

Thus, the two substrates of this enzyme are pimeloyl-CoA and NAD⁺, whereas its 3 products are 6-carboxyhex-2-enoyl-CoA, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is pimeloyl-CoA:NAD+ oxidoreductase. This enzyme participates in benzoate degradation via coa ligation.

• Gallus C, Schink B (1994). "Anaerobic degradation of pimelate by newly isolated denitrifying bacteria". Microbiology. 140 (2): 409–16. doi:10.1099/13500872-140-2-409. PMID 8180704.