O Negative refers to blood group O, Rh-negative, in the ABO and Rh blood group systems.
O Negative may also refer to:
- O−, an oxygen ion with a charge of -1
- O-Negative (1998 film), a 1998 Thai film
- O Negative (2015 film), a 2015 Canadian short film
- Type O Negative, an American heavy metal band
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