In enzymology, a N-acylhexosamine oxidase (EC 1.1.3.29) is an enzyme that catalyzes the chemical reaction

N-acetyl-D-glucosamine + O₂ N-acetyl-D-glucosaminate + H₂O₂

Thus, the two substrates of this enzyme are N-acetyl-D-glucosamine and O₂, whereas its two products are N-acetyl-D-glucosaminate and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is N-acyl-D-hexosamine:oxygen 1-oxidoreductase. Other names in common use include N-acyl-D-hexosamine oxidase, and N-acyl-beta-D-hexosamine:oxygen 1-oxidoreductase.

• Horiuchi T (1989). "Purification and properties of N-acyl-D-hexosamine oxidase from Pseudomonas sp 15-1". Agric. Biol. Chem. 53 (2): 361–368. doi:10.1271/bbb1961.53.361.
• Rembeza E, Boverio A, Fraaije M, Engqvist M (2021). "Discovery of two novel oxidases using a high-throughput activity screen". ChemBioChem. doi:10.1002/cbic.202100510.