The following highways are numbered 54:

• Alberta Highway 54
• Saskatchewan Highway 54

• Finnish national road 54

• EO54 road

• National Highway 54 (India)

• Autostrada A54

• Japan National Route 54

• National Route 54

• Mexican Federal Highway 54

• New Zealand State Highway 54

• N54 highway (Philippines)

• O-54, a motorway in Turkey as the half ring road in Gaziantep.

• British A54 (Buxton-Chester)
• British M54 (Wellington-Essington)

• U.S. Route 54
• Alabama State Route 54
• Arkansas Highway 54
• California State Route 54
• Delaware Route 54
• Florida State Road 54
  • County Road 54 (Pasco County, Florida)
• Georgia State Route 54
  • Georgia State Route 54B (former)
• Idaho State Highway 54
• Illinois Route 54
• Indiana State Road 54
• Kentucky Route 54
• Louisiana Highway 54
• Maryland Route 54
• M-54 (Michigan highway)
• Minnesota State Highway 54
  • County Road 54 (Anoka County, Minnesota)
• Missouri Route 54 (1922) (former)
• Nebraska Highway 54 (former)
  • Nebraska Spur 54B
  • Nebraska Spur 54D
  • Nebraska Recreation Road 54C
  • Nebraska Recreation Road 54F
• Nevada State Route 54 (former)
• New Jersey Route 54
  • County Route 54 (Bergen County, New Jersey)
  • County Route 54 (Monmouth County, New Jersey)
• New York State Route 54
  • County Route 54 (Cayuga County, New York)
  • County Route 54 (Chautauqua County, New York)
  • County Route 54 (Chemung County, New York)
  • County Route 54 (Dutchess County, New York)
  • County Route 54 (Franklin County, New York)
  • County Route 54 (Jefferson County, New York)
  • County Route 54 (Lewis County, New York)
  • County Route 54 (Orange County, New York)
  • County Route 54 (Oswego County, New York)
  • County Route 54 (Otsego County, New York)
  • County Route 54 (Putnam County, New York)
  • County Route 54 (Rensselaer County, New York)
  • County Route 54 (Saratoga County, New York)
  • County Route 54 (Schoharie County, New York)
  • County Route 54 (St. Lawrence County, New York)
  • County Route 54 (Suffolk County, New York)
  • County Route 54 (Tioga County, New York)
  • County Route 54 (Ulster County, New York)
  • County Route 54 (Washington County, New York)
  • County Route 54 (Westchester County, New York)
  • County Route 54 (Wyoming County, New York)
• North Carolina Highway 54
• North Dakota Highway 54
• Ohio State Route 54
• Oklahoma State Highway 54
• Pennsylvania Route 54
• South Dakota Highway 54 (former)
• Tennessee State Route 54
• Texas State Highway 54
  • Texas Park Road 54
• Utah State Route 54
  • Utah State Route 54 (1931-1969) (former)
• Virginia State Route 54
• West Virginia Route 54
• Wisconsin Highway 54

Territories

• Puerto Rico Highway 54
  • Puerto Rico Highway 54R

• List of highways numbered 54A
• A54