Route 431, or Highway 431, may refer to:

• Newfoundland and Labrador Route 431

• Callejero de Reparto Universitario (4–431)

• Main road 431 (Hungary)

• National Highway 431 (India)

• Highway 431 (Israel)

• Japan National Route 431

• U.S. Route 431
• Florida:
  • Florida State Road 431
    • Florida State Road 431B (former)
  • County Road 431 (Seminole County, Florida)
  • County Road 431B (Seminole County, Florida)
• Indiana State Road 431 (former)
• Iowa Highway 431 (former)
• Nevada State Route 431
• New York State Route 431
• Puerto Rico Highway 431
• Tennessee State Route 431
• Texas:
  • Texas State Highway Loop 431
  • Farm to Market Road 431
• Washington State Route 431
• Wyoming Highway 431