Route 264 or Highway 264 may refer to:

• Manitoba Provincial Road 264
• Prince Edward Island Route 264
• Saskatchewan Highway 264

• Route 264 (Israel)

• Japan National Route 264

• Interstate 264 (multiple highways)
• U.S. Route 264
• Arizona State Route 264
• Arkansas Highway 264
• Georgia State Route 264
• Indiana State Road 264
• K-264 (Kansas highway)
• Maryland Route 264
• Minnesota State Highway 264
• Montana Secondary Highway 264
• Nevada State Route 264
• New Mexico State Road 264
• New York State Route 264
• Ohio State Route 264
• Pennsylvania Route 264 (former)
• South Dakota Highway 264 (former)
• Tennessee State Route 264
• Texas State Highway 264 (former)
  • Texas State Highway Spur 264
  • Farm to Market Road 264 (Texas)
• Utah State Route 264
• Virginia State Route 264