John Boys may refer to:

• John Boys (fl. 1388), MP for Plympton Erle (UK Parliament constituency)
• John Boys (died 1447), MP for Middlesex (UK Parliament constituency) and Hampshire
• John Boys (died 1612), MP for Canterbury, Sandwich and Midhurst
• John Boys (died 1533), MP for Sandwich (UK Parliament constituency)
• John Boys (Parliamentarian) (c. 1607–1678), MP for Kent

• John Boys (priest) (1571–1625), Dean of Canterbury, 1619–1625
• John Boys (bishop) (1897–1965), Bishop of Kimberley and Kuruman, 1951–1960

• John Bois (1560–1643), also spelt John Boys, English translator
• John Boys (classicist) (1614?–1661), English translator of Virgil

• John Boys (Royalist) (1607–1664), military commander
• John Boys (agriculturalist) (1749–1824), agriculturist
• John Boys (cricketer) (1856–1883), English cricketer

• John Boyes (disambiguation)
• John Boy and Billy