James Bolton (1735–1799) was an English naturalist and illustrator.

James Bolton may also refer to:

• James W. Bolton (1869–1936), American banker, civic leader, and school board president
• James C. Bolton (1899–1974), American banker, civic leader, and Baptist layman, son of James W. Bolton
• James Bolton (footballer) (born 1994), English footballer
• Jim Bolton (historian), English medieval economic historian

In fiction:

• James "Jambo" Bolton, Hollyoaks character
• James Bolton, character in The Love Hermit

• All pages with titles containing James Bolton