Holstein may refer to:

• Holstein Friesian, a cattle breed used in dairy farming
• Holsteiner, a breed of horse originating in the Schleswig-Holstein region of northern Germany

• Schleswig-Holstein, a state in Northern Germany
• Holstein, a region in Germany
• Duchy of Holstein
• Holstein, Ontario, a village in Canada
• New Holstein, Wisconsin, United States
• Holstein, Iowa, United States
• Holstein, Missouri, United States
• Holstein, Nebraska, United States
• the German name of Pregolskiy, Kaliningrad, Russia
• Hölstein, a village in Basel-Landschaft, Switzerland

n.b. In Danish, the spelling of Holstein can be varied as Holsten or Holsteen

• Holsten (I) – a ship of the Danish East India Company (1800–1805); originally HDMS Det Store Bælt (1782)
• Holsten (II) – a ship of the Danish East India Company (1806–1808); see Warren Hastings (1802 EIC ship)
• HDMS Holsteen, a Danish ship-of-the-line

• Holstein (station), an Oslo Metro station
• Holstein (surname)
• Holstein interglacial, a geologic stretch of time
• Holstein-Primakoff transformation, a mathematical physics transformation
• Pedro de Sousa Holstein, 1st Duke of Palmela (1781–1850), Portuguese diplomat and statesmen

• Holston (disambiguation)