The enzyme glucose-1-phosphatase (EC 3.1.3.10) catalyzes the reaction

α-D-glucose 1-phosphate + H₂O D-glucose + phosphate

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name is α-D-glucose-1-phosphate phosphohydrolase. This enzyme participates in glycolysis and gluconeogenesis.

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1NT4.

• Faulkner P (August 1955). "A hexose-1-phosphatase in silkworm blood". The Biochemical Journal. 60 (4): 590–6. doi:10.1042/bj0600590. PMC 1216157. PMID 13249953.
• Turner DH, Turner JF (March 1960). "The hydrolysis of glucose monophosphates by a phosphatase preparation from pea seeds". The Biochemical Journal. 74 (3): 486–91. doi:10.1042/bj0740486. PMC 1204246. PMID 13839934.