The enzyme GDP-glucosidase (EC 3.2.1.42) catalyzes the chemical reaction

GDP-glucose + H₂O D-glucose + GDP

Thus, the two substrates of this enzyme are GDP-glucose and H₂O, whereas its two products are D-glucose and GDP.

This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is GDP-glucose glucohydrolase. Other names in common use include guanosine diphosphoglucosidase, and guanosine diphosphate D-glucose glucohydrolase.

• Sonnino S, Carminatti H, Cabib E (1966). "Guanosine diphosphate D-glucose glucohydrolase". Arch. Biochem. Biophys. 116 (1): 26–33. doi:10.1016/0003-9861(66)90007-5. PMID 5963308.