Erythromycin 3''-O-methyltransferase (EC 2.1.1.254, EryG) is an enzyme with systematic name S-adenosyl-L-methionine:erythromycin C 3''-O-methyltransferase.[1][2] This enzyme catalyses the following chemical reaction

(1) S-adenosyl-L-methionine + erythromycin C S-adenosyl-L-homocysteine + erythromycin A
(2) S-adenosyl-L-methionine + erythromycin D S-adenosyl-L-homocysteine + erythromycin B

The enzyme methylates the 3 position of the mycarosyl moiety of erythromycin C.

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