Closing time may refer to:

• Closing Time (novel), a 1994 novel and sequel to Catch-22 by Joseph Heller
• Closing Time: The True Story of the Goodbar Murder, a 1977 book by Lacey Fosburgh
• "Closing Time", a short story by Neil Gaiman included in the 2006 collection Fragile Things

• Closing Time (album), a 1973 album by Tom Waits, or the title song
• "Closing Time" (Deacon Blue song), 1991
• "Closing Time" (Hole song), 1993
• "Closing Time" (Semisonic song), 1998
• "Closing Time", a song by Leonard Cohen from The Future, 1992
• "Closing Time", a song by Lyle Lovett from Lyle Lovett, 1986
• "Closing Time", a song by Tyler Joe Miller from Spillin' My Truth, 2023
• "Lounge (Closing Time)", a song by Modest Mouse from The Lonesome Crowded West, 1997

• "Closing Time" (Beavis and Butt-head)
• "Closing Time" (Doctor Who)
• "Closing Time" (Two Pints of Lager and a Packet of Crisps)

• Closing time effect
• Business hours
• Last call (disambiguation)