Ayacucho is the capital city of Huamanga Province, Ayacucho Region, Peru

Ayacucho may also refer to:

• Ayacucho, Buenos Aires, a city in Buenos Aires Province
• Ayacucho Partido, an administrative division of Buenos Aires Province
• Ayacucho Department, San Luis, an administrative division of San Luis Province

• Ayacucho Region, an administrative division
• Ayacucho District, a district within the region
• Ayacucho Quechua, the Quechua dialect spoken in the Ayacucho Region
• Ayacucho metro station, a rapid transit station in Lima
• Battle of Ayacucho, a decisive battle in the War of Independence, 9 December 1824

• Puerto Ayacucho, a city in Venezuela
• Ayacucho (crater), an impact crater on Mars
• Ayacucho Parish, a parish in Guayaquil, Guayas, Ecuador