Anne Stuart may refer to:

• Anne, Countess of Auvergne (1496–1524), married James Stewart, duke of Albany
• Anne of Denmark (1574–1619), wife of James I of Great Britain (VI of Scotland)

• Anne Stuart (born 1637), daughter of Charles I of Great Britain
• Anne Hyde (1638–1671), married name Anne Stuart, wife of James II of Great Britain (VII of Scotland) and mother of Mary II and Queen Anne
• Anne, Queen of Great Britain (1665–1714)

• Anne Stuart Percy, Lady Warkworth (c. 1745–1813)

• Anne Mackenzie-Stuart (1930–2008), activist
• Anne Stuart (novelist) (born 1948), American romance novelist
• Ann Stuart, chancellor and president of Texas Woman's University
• Ann Stuart (scientist), neurophysiologist at UNC Chapel Hill

• Amelia Stewart, Viscountess Castlereagh (1772–1829), member of Regency London high society
• Anna Stewart (disambiguation)