First-seeded Margaret Smith was the four-time defending champion, and successfully defended her title, defeating Lesley Turner 6–3, 6–2 in the final to win the women's singles tennis title at the 1964 Australian Championships.

The seeded players are listed below. Margaret Smith is the champion; others show the round in which they were eliminated.

1. Margaret Smith(champion)
2. Lesley Turner (finalist)
3. Jan Lehane (semifinals)
4. Robyn Ebbern (semifinals)
5. Judy Tegart (quarterfinals)
6. Madonna Schacht (quarterfinals)
7. Jill Blackman (quarterfinals)
8. Rita Bentley (first round)

• Q = Qualifier
• WC = Wild card
• LL = Lucky loser
• r = Retired

	Quarterfinals							Semifinals							Finals
	1	Margaret Smith	4	6	6
	6	Madonna Schacht	6	1	1
								1	Margaret Smith	6	6
								3	Jan Lehane	4	2
	3	Jan Lehane	6	6
		Helen Gourlay	2	0
															1	Margaret Smith	6	6
															2	Lesley Turner	3	2
	5	Judy Tegart	2	4
	4	Robyn Ebbern	6	6
								4	Robyn Ebbern	3	1
								2	Lesley Turner	6	6
	7	Jill Blackman	1	3
	2	Lesley Turner	6	6

	First round							Second round							Quarterfinals							Semifinals
								1	Smith	6	6
		Hawton	6	6					Hawton	1	0
		Krantzcke	3	3											1	Smith	4	6	6
	6	Schacht	6	6											6	Schacht	6	1	1
		Boucaut	0	1				6	Schacht	6	5	6
		Whitely	6	6					Whitely	4	7	4
		Sherriff	1	2																		1	Smith	6	6
																						3	Lehane	4	2
								3	Lehane	6	6
		Melville	6	6					Melville	2	0
		Van Haren	2	4											3	Lehane	6	6
		Dening	2	6	6											Gourlay	2	0
	8	Bentley	6	1	3				Dening	4	4
		Gourlay	3	6	6				Gourlay	6	6
		Starr	6	3	0

	First round							Second round							Quarterfinals							Semifinals
		Sherriff	6	6
		Toyne	1	0					Sherriff	3	6	2
								5	Tegart	6	3	6
															5	Tegart	2	4
		Gibson	7	6											4	Ebbern	6	6
		Miyagi	5	2					Gibson	6	6	4
								4	Ebbern	4	8	6
																						4	Ebbern	3	1
		Jenkins	7	4	6																	2	Turner	6	6
		Turner	5	6	3				Jenkins	4	2
	7	Blackman	6	6				7	Blackman	6	6
		Bellamy	1	3											7	Blackman	1	3
		Kynaston	6	6											2	Turner	6	6
		Eastburn	2	2					Kynaston	1	2
								2	Turner	6	6

• 1964 Australian Championships on australianopen.com, the source for this draw