The enzyme [acyl-carrier-protein] phosphodiesterase (EC 3.1.4.14) catalyzes the reaction

holo-[acyl-carrier-protein] + H₂O 4′-phosphopantetheine + apo-[acyl-carrier-protein]

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric diester bonds. The systematic name is holo-[acyl-carrier-protein] 4′-pantetheine-phosphohydrolase. Other names in common use include ACP hydrolyase, ACP phosphodiesterase, AcpH, and [acyl-carrier-protein] 4′-pantetheine-phosphohydrolase. This enzyme participates in pantothenate and CoA biosynthesis.

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1T5B and 1TIK.

• Sobhy C (1979). "Regulation of fatty acid synthetase activity. The 4′-phosphopantetheine hydrolase of rat liver". J. Biol. Chem. 254 (17): 8561–6. doi:10.1016/S0021-9258(19)86929-6. PMID 224058.
• Vagelos PR, Larrabes AR (1967). "Acyl carrier protein. IX. Acyl carrier protein hydrolase". J. Biol. Chem. 242 (8): 1776–81. doi:10.1016/S0021-9258(18)96069-2. PMID 4290442.
• Thomas J, Cronan JE (2005). "The enigmatic acyl carrier protein phosphodiesterase of Escherichia coli: genetic and enzymological characterization". J. Biol. Chem. 280 (41): 34675–83. doi:10.1074/jbc.M505736200. PMID 16107329.