Parthenos (Greek: παρθένος 'virgin') may refer to:

• Virgin (title), an honorific bestowed on female saints and blesseds in Christianity
• Parthenos, the name of several maidens in the history of the naming of the constellation Virgo
• Parthenos, one translation of Hebrew almah
  • Isaiah 7:14, a verse in the bible
• Parthenos (butterfly)

• Artemis, in Greek mythology
• Athena, in Greek mythology
  • Athena Parthenos, a monumental sculpture
• Hestia, in Greek mythology
• Aeiparthenos, the perpetual virginity of Mary
• O Virgin Pure, a Greek Marian hymn