Route 28 is a highway in central Missouri. Its eastern terminus is at U.S. Route 50 in eastern Gasconade County; its western terminus is at Interstate 44 east of St. Robert. Route 28 was one of the original state highways and it has changed very little. Its termini are currently the same as they were in 1922.

County	Location	mi	km	Destinations	Notes
Pulaski	St. Robert			Route Z / Historic US 66
				I-44 – Springfield, Fort Leonard Wood, Rolla	I-44 exit 163
	Dixon			Route C west to Route 133
Maries	​			US 63 north – Vienna, Westphalia, Jefferson City	west end of US 63 overlap
	​			US 63 south – Vichy, Rolla, Houston	east end of US 63 overlap
	​			Route 42 west – Vienna
	Belle			Route 89 north – Linn, Chamois
Gasconade	Owensville			Route 19 south – Cuba	west end of Route 19 overlap
	​			Route 19 north – Drake	east end of Route 19 overlap
	Rosebud			US 50 – Jefferson City, St. Louis, Drake, Gerald
1.000 mi = 1.609 km; 1.000 km = 0.621 mi