Lifeblood or Life blood can refer to:

• Australian Red Cross Lifeblood, the organisation responsible for blood and related donations in Australia
• Blood, a bodily fluid in animals
• Life Blood (film), a supernatural horror thriller film released in 2009
• Lifeblood (album), the seventh studio album by the Welsh alternative rock band Manic Street Preachers
• Lifesblood, a 2001 EP by Mastodon
• Lifeblood (novel), sequel to the novel Darkside, a children's novel by Tom Becker
• Life's Blood, an American hardcore punk band
• "Life's Blood", a song by the Eighteen Visions from their EP Lifeless and album The Best of Eighteen Visions
• Lifeblood (charity), a UK-based charity now known as Thrombosis UK
• Lifeblood, 2021 studio album by Brand of Sacrifice