Ayrshire is a registration county, and former administrative county in south-west Scotland, United Kingdom. Ayrshire may also refer to:

• Shire of Ayr, Queensland, Australia

• Ayrshire (constituency), a list of places in the United Kingdom
• Ayrshire (UK Parliament constituency), a county constituency of the House of Commons of the Parliaments of Great Britain and the United Kingdom from 1708 to 1868
• Ayrshire (Parliament of Scotland constituency)
• Ayrshire and Arran, a lieutenancy area in Scotland

• Ayrshire, Indiana an unincorporated community in Pike County, Indiana
• Ayrshire, Iowa, a city in Palo Alto County, Iowa

• Ayrshire Apartments, Polk County, Iowa, USA; listed on the National Register of Historic Places
• Ayrshire College, Ayrshire, Scotland, UK; a college
• Ayrshire Central Hospital, Irvine, North Ayrshire, Ayrshire, Scotland, UK

• Ayrshire (Earl of Carrick's Own) Yeomanry, of the British Army
• Ayrshire Royal Horse Artillery, a volunteer force supplementing the British Army

• Ayrshire (horse), a British Thoroughbred racehorse
• Ayrshire Cup, annual soccer competition in Scotland
• Ayrshire Football Association, multiple leagues

• Ayrshire cattle, a breed of dairy cattle originating from Ayrshire in Scotland
• Lord Lieutenant of Ayrshire

• All pages with titles containing ayrshire or ayrshires
• All pages with titles beginning with Ayrshire
• Central Ayrshire, Ayrshire, Scotland, UK
• East Ayrshire, Ayrshire, Scotland, UK
• North Ayrshire, Ayrshire, Scotland, UK
• South Ayrshire, Ayrshire, Scotland, UK
• Ayr (disambiguation)
• Ayr County (disambiguation)
• Ayr Township (disambiguation)
• Ayre (disambiguation)
• Ayrshire Hospital (disambiguation)