The 2023 Lexington Challenger was a professional tennis tournament played on outdoor hard courts. It was the 28th edition of the tournament which was part of the 2023 ATP Challenger Tour, and the 26th edition of the tournament which was part of the 2023 ITF Women's World Tennis Tour. It took place in Lexington, Kentucky, United States between 31 July and 6 August 2023.

• Steve Johnson def. Arthur Cazaux 7–6^(7–5), 6–4.

• Eliot Spizzirri / Tyler Zink def. George Goldhoff / Vasil Kirkov 4–6, 6–3, [10–8].

• Renata Zarazúa def. Caroline Dolehide 1–6, 7–6^(7–4), 7–5.

• Alexis Blokhina / Ava Markham def. Olivia Gadecki / Dalayna Hewitt 6–4, 7–6^(7–1).

Country	Player	Rank1	Seed
FRA	Arthur Cazaux	141	1
USA	Steve Johnson	190	2
TPE	Wu Tung-lin	195	3
KOR	Hong Seong-chan	206	4
USA	Tennys Sandgren	214	5
AUS	Dane Sweeny	230	6
AUS	Adam Walton	247	7
AUS	Tristan Schoolkate	264	8

• ¹ Rankings as of 24 July 2023.^[1]

The following players received a wildcard into the singles main draw:

• Martin Damm
• Cannon Kingsley
• Joshua Lapadat

The following player received entry into the singles main draw using a protected ranking:

• Christian Harrison

The following players received entry into the singles main draw as alternates:

• Ulises Blanch
• Nathan Ponwith

The following players received entry from the qualifying draw:

• Justin Boulais
• Tristan Boyer
• Stefan Dostanic
• Aidan Mayo
• Yuki Mochizuki
• Philip Sekulic

Country	Player	Rank1	Seed
USA	Caroline Dolehide	102	1
USA	Madison Brengle	103	2
AUS	Kimberly Birrell	111	3
USA	Katie Volynets	126	4
AUS	Olivia Gadecki	144	5
GBR	Katie Swan	148	6
JPN	Mai Hontama	179	7
CHN	Wang Yafan	182	8

• ¹ Rankings as of 24 July 2023.

The following players received a wildcard into the singles main draw:

• Elizabeth Eades
• Dalayna Hewitt
• McCartney Kessler
• Madison Sieg

The following players received entry from the qualifying draw:

• Chloe Beck
• DJ Bennett
• Samantha Crawford
• Sara Daavettila
• Tatum Evans
• Victoria Hu
• Grace Min
• Christina Rosca