Wolfsbane may refer to:

• Aconitum, a genus of toxic flowering plants of the northern hemisphere
  • Aconitum lycoctonum or northern wolfsbane
• Arnica montana, a toxic European flowering plant

• Wolfsbane (band), an English heavy metal/hard rock band
  • Wolfsbane (album), 1994
• Wolfsbane (character), a Marvel Comics superhero
• Wolfsbane (novel), a 2003 Doctor Who novel by Jacqueline Rayner
• Wolfbane (novel), a 1959 novel by Frederik Pohl and C. M. Kornbluth
• Wolfbane, a 2022 novel in Michelle Paver's Chronicles of Ancient Darkness series
• Wolfsbane, a 1978 novel by Craig Thomas
• Wolf's Bane, a 1993 fantasy book set in the Lone Wolf universe
• Sir Peter Wolf's-Bane, a title Aslan gives to Peter Pevensie in C. S. Lewis' The Lion, the Witch and the Wardrobe novel after he kills Maugrim, a talking wolf